evaluation of metallo-β-lactamase-production and carriage of bla-vim genes in pseudomonas aeruginosa isolated from burn wound infections in isfahan

نویسندگان

mahmood saffari trauma research center, kashan university of medical sciences, kashan, ir iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی کاشان (kashan university of medical sciences)

farzaneh firoozeh department of microbiology and immunology, school of medicine, kashan university of medical sciences, kashan, ir iran; department of microbiology and immunology, p. o. box: 87159-88141, kashan, ir iran. tel/fax: +98-3155540021سازمان اصلی تایید شده: دانشگاه علوم پزشکی کاشان (kashan university of medical sciences)

mohammad pourbabaee department of microbiology and immunology, school of medicine, kashan university of medical sciences, kashan, ir iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی کاشان (kashan university of medical sciences)

mohammad zibaei department of parasitology and mycology, school of medicine, alborz university of medical sciences, karaj, ir iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی البرز (alborz university of medical sciences)

چکیده

conclusions our findings showed a high occurrence of metallo-β-lactamase production among p. aeruginosa isolates in burn patient infections in our region. also, there are p. aeruginosa isolates carrying the bla-vim-1 and bla-vim-2 genes in isfahan province. background metallo-β-lactamase-production among gram-negative bacteria, including pseudomonas aeruginosa, has become a challenge for treatment of infections due to these resistant bacteria. objectives the aim of the current study was to evaluate the metallo-β-lactamase-production and carriage of bla-vim genes among carbapenem-resistant p. aeruginosa isolated from burn wound infections. patients and methods a cross-sectional study was conducted from september 2014 to july 2015. one hundred and fifty p. aeruginosa isolates were recovered from 600 patients with burn wound infections treated at imam-musa-kazem hospital in isfahan city, iran. carbapenem-resistant p. aeruginosa isolates were screened by disk diffusion using clsi guidelines. metallo-β-lactamase-producing p. aeruginosa isolates were identified using an imipenem-edta double disk synergy test (edta-imp ddst). for detection of mbl genes including bla-vim-1 and bla-vim-2, polymerase chain reaction (pcr) methods and sequencing were used. results among the 150 p. aeruginosa isolates, 144 (96%) were resistant to imipenem by the disk diffusion method, all of which were identified as metallo-β-lactamase-producing p. aeruginosa isolates by edta-imp ddst. twenty-seven (18%) and 8 (5.5%) mbl-producing p. aeruginosa isolates harbored bla-vim-1 and bla-vim-2 genes, respectively.

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Evaluation of Metallo-β-Lactamase-Production and Carriage of bla-VIM Genes in Pseudomonas aeruginosa Isolated from Burn Wound Infections in Isfahan

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عنوان ژورنال:
archives of trauma research

جلد ۵، شماره ۴، صفحات ۰-۰

کلمات کلیدی
[ ' b u r n p a t i e n t s ' , ' m e t a l l o ' , ' β ' , ' l a c t a m a s e ' , ' b l a v i m ' , 1 , ' b l a v i m ' , 1 , ' p s e u d o m o n a s a e r u g i n o s a ' ]

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